Sequence optimization based on STARR-seq models.
Legacy models are included for reproducibility.
Paste a single 170 bp DNA sequence. FASTA headers and line breaks are allowed.
Number of optimization rounds (1–25).
Add one or more conditions and target values. PETAL will select mutations that minimize the summed absolute distance to all selected target values.
Select a model first, then add one or more evolution objectives.
Optional email delivery for the PETAL results (.tsv).
The plot below shows predicted STARR-seq activity across evolution rounds.
Note: In silico evolution may introduce unwanted sequence motifs. You can remove these while minimizing changes to predicted activity with PETAL.Domesticate .
Don’t forget to cite this model and the PETAL suite: